ARG83437
Human Cytochrome C ELISA Kit
Human Cytochrome C ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | ARG83437 Human Cytochrome C ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Cytochrome C in Cell Lysates |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Cytochrome C |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 40 pg/ml |
| 样品类型 | Cell Lysates |
| 标准范围 | 78 - 5000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: less than 10% Inter-Assay CV: less than 10% |
| 別名 | CYC; HCS; Cytochrome c; THC4 |
应用说明
| 检测时间 | 3.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 4°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CYCS |
| 全名 | cytochrome c, somatic |
| 背景介绍 | Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases. |
| 生物功能 | Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. [UniProt] |
| 细胞定位 | Mitochondrion |
| 翻译后修饰 | Acetylation, Phosphoprotein |
检测图片 (1) Click the Picture to Zoom In
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ARG83437 Human Cytochrome C ELISA Kit standard curve image
ARG83437 Human Cytochrome C ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83437 Human Cytochrome C ELISA Kit User manual |
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