ARG81914
Human COMT ELISA Kit
Human COMT ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81914-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81914-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81914-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81914-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81914-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81914-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81914-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81914-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81914-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81914-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81914-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81914 Human COMT ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human COMT in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | COMT |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.8%; Inter-Assay CV: 7.9% |
| 別名 | EC 2.1.1.6; Catechol O-methyltransferase; HEL-S-98n |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | COMT |
| 全名 | catechol-O-methyltransferase |
| 背景介绍 | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008] |
| 生物功能 | Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. [UniProt] |
| 产品亮点 | Related products: COMT antibodies; COMT ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The N-terminus is blocked. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81914 Human COMT ELISA Kit standard curve image
ARG81914 Human COMT ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81914 Human COMT ELISA Kit User manual |
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