ARG70267
Human CD172a / SIRP alpha recombinant protein (Fc-His-tagged, C-ter)
Human CD172a / SIRP alpha recombinant protein (Fc-His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Human CD172a / SIRP alpha recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | CD172a / SIRP alpha |
| 物种 | Human |
| A.A. 序列 | Glu31 - Arg370 of Human CD172a / SIRP alpha (NP_542970.1) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | CD172A; p84; SHPS1; SHPS-1; CD172 antigen-like family member A; Sirp-alpha-3; Sirp-alpha-1; BIT; MYD-1; MFR; Bit; PTPNS1; CD antigen CD172a; Inhibitory receptor SHPS-1; SIRP; MyD-1 antigen; Sirp-alpha-2; Tyrosine-protein phosphatase non-receptor type substrate 1; Signal-regulatory protein alpha-1; Signal-regulatory protein alpha-2; Signal-regulatory protein alpha-3; Macrophage fusion receptor; Brain Ig-like molecule with tyrosine-based activation motifs; P84; SHP substrate 1 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human CD47 at 1 µg/ml (100 µl/well) can bind Recombinant Human SIRP alpha with a linear range of 1-6 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | SIRPA |
|---|---|
| 全名 | signal-regulatory protein alpha |
| 背景介绍 | The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 55 kDa |
| 翻译后修饰 | N-glycosylated. Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70267 Human CD172a / SIRP alpha recombinant protein (ECD) (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70267 Human CD172a / SIRP alpha recombinant protein (ECD) (Fc-His-tagged, C-ter).
