ARG82856
Human CA3 / Carbonic Anhydrase 3 ELISA Kit
Human CA3 / Carbonic Anhydrase 3 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82856-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82856-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82856-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82856-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82856-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82856-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82856-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82856-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82856-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82856-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82856-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA3 / Carbonic Anhydrase 3 in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | CA3 / Carbonic Anhydrase 3 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.39 ng/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
| 标准范围 | 0.78 - 50 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.9% Inter-Assay CV: 5.5% |
| 別名 | CAIII; EC 4.2.1.1; Car3; CA-III; Carbonate dehydratase III; Carbonic anhydrase 3; Carbonic anhydrase III |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CA3 |
| 全名 | carbonic anhydrase III |
| 背景介绍 | Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008] |
| 生物功能 | Reversible hydration of carbon dioxide. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 翻译后修饰 | S-glutathionylated in hepatocytes under oxidative stress. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit standard curve image
ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82856 Human CA3 / Carbonic Anhydrase 3 ELISA Kit User manual |
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