ARG82360
Human CA2 / Carbonic Anhydrase 2 ELISA Kit
Human CA2 / Carbonic Anhydrase 2 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82360-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82360-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82360-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82360-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82360-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82360-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82360-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82360-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82360-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82360-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82360-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82360 Human CA2 / Carbonic Anhydrase 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human CA2 / Carbonic Anhydrase 2 in serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | CA2 / Carbonic Anhydrase 2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.39 ng/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate), cell culture supernatants and urine. |
| 标准范围 | 0.78 - 50 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.3% Inter-Assay CV: 5.3% |
| 別名 | Carbonic anhydrase C; EC 4.2.1.1; Car2; Carbonate dehydratase II; CA-II; Carbonic anhydrase II; CAC; HEL-76; Carbonic anhydrase 2; CAII |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CA2 |
| 全名 | carbonic anhydrase II |
| 背景介绍 | The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014] |
| 生物功能 | Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. [UniProt] |
| 细胞定位 | Cytoplasm. Cell membrane. Note=Colocalized with SLC26A6 at the surface of the cell membrane in order to form a bicarbonate transport metabolon. Displaced from the cytosolic surface of the cell membrane by PKC in phorbol myristate acetate (PMA)-induced cells. [UniProt] |
| 产品亮点 | Related products: Carbonic Anhydrase antibodies; Carbonic Anhydrase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82360 Human CA2 / Carbonic Anhydrase 2 ELISA Kit standard curve image
ARG82360 Human CA2 / Carbonic Anhydrase 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82360 Human CA2 / Carbonic Anhydrase 2 ELISA Kit User manual |
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