Human BDNF recombinant protein (Active) (His-tagged, C-ter)

Human BDNF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE


产品描述 E. coli expressed, His-tagged (C-ter) Active Human BDNF recombinant protein
靶点名称 BDNF
物种 Human
A.A. 序列 His132 - Arg250
表达系统 E. coli
生物活性 Active
活性说明 Determined by its ability to induce proliferation in BaF3 cells transfected with TrkB. The ED50 for this effect is <2 ng/mL.
別名 Brain-derived neurotrophic factor; Abrineurin; BULN2; ANON2; BDNF


形式 Powder
纯化说明 Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
纯度 >98% (by SDS-PAGE)
缓冲液 20 mM sodium citrate and 0.2 M NaCl (pH 3.5)
复溶 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
存放说明 For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事项 For laboratory research only, not for drug, diagnostic or other use.


基因名称 BDNF
全名 brain-derived neurotrophic factor
背景介绍 The protein encoded by this gene is a member of the nerve growth factor family. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer's and Huntington disease patients. This gene may play a role in the regulation of stress response and in the biology of mood disorders. Multiple transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Jan 2009]
生物功能 During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. [UniProt]
细胞定位 Secreted. [UniProt]
产品亮点 Related products:
BDNF antibodies; BDNF ELISA Kits; BDNF recombinant proteins;
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翻译后修饰 The propeptide is N-glycosylated and glycosulfated.

Converted into mature BDNF by plasmin (PLG). [UniProt]

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  • ARG70141 Human BDNF recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70141 Human BDNF recombinant protein (Active) (His-tagged, C-ter).