ARG81477
Human Aggrecan ELISA Kit
Human Aggrecan ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81477-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81477-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG81477-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81477-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81477-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81477-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81477-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81477-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81477-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81477-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81477-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81477 Human Aggrecan ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Aggrecan in serum, urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ACAN / Aggrecan |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.78 ng/ml |
| 样品类型 | Serum, urine and cell culture supernatants. |
| 标准范围 | 1.56 - 100 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8% Inter-Assay CV: 6.4% |
| 別名 | AGC1; SEDK; AGCAN; CSPG1; MSK16; CSPGCP; SSOAOD |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | ACAN |
|---|---|
| 全名 | aggrecan |
| 背景介绍 | This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. [UniProt] |
| 产品亮点 | Related products: ACAN antibodies; ACAN ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Contains mostly chondroitin sulfate, but also keratan sulfate chains, N-linked and O-linked oligosaccharides. The release of aggrecan fragments from articular cartilage into the synovial fluid at all stages of human osteoarthritis is the result of cleavage by aggrecanase. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81477 Human Aggrecan ELISA Kit standard curve image
ARG81477 Human Aggrecan ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81477 Human Aggrecan ELISA Kit User's manual |
 Download
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