ARG83231
Human ASAH2 ELISA Kit
Human ASAH2 ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | ARG83231 Human ASAH2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ASAH2 in Serum, Plasma and Cell culture supernatants. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ASAH2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15 pg/ml |
| 检测范围 | 62.5 pg/ml - 4,000 pg/ml |
| 样品类型 | Serum, Plasma and Cell culture supernatants |
| 精确 | Intra-Assay CV: 6.7% Inter-Assay CV: 5.9% |
| 別名 | hCD; EC 3.5.1.23; HNAC1; LCDase; Acylsphingosine deacylase 2; Neutral ceramidase; NCDase; Non-lysosomal ceramidase; BCDase; N-CDase; N-acylsphingosine amidohydrolase 2 |
应用说明
| 检测时间 | ~ 5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | ASAH2 |
| 全名 | N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2 |
| 背景介绍 | Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008] |
| 生物功能 | Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. [UniProt] |
| 翻译后修饰 | N-glycosylated. Required for enzyme activity (By similarity). O-glycosylated. Required to retain it as a type II membrane protein at the cell surface. Phosphorylated. May prevent ubiquitination and subsequent degradation (By similarity). Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is triggered by nitric oxid (By similarity). |
检测图片 (1) Click the Picture to Zoom In
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ARG83231 Human ASAH2 ELISA Kit standard curve image
ARG83231 Human ASAH2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83231 Human ASAH2 ELISA Kit User manual |
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