ARG82404

Human ARMET ELISA Kit

Human ARMET ELISA 试剂盒 for ELISA and Human

内含物

货号 内含物名称 包装 温度
ARG82404-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82404-002 Standard 2 X 10 ng/vial 4°C
ARG82404-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82404-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82404-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82404-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82404-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82404-008 25X Wash buffer 20 ml 4°C
ARG82404-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82404-010 STOP solution 10 ml (Ready to use) 4°C
ARG82404-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG82404 Human ARMET ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ARMET in serum, plasma (EDTA, heparin) and cell culture supernatants.
反应物种 Hu
应用 ELISA
靶点名称 ARMET
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 46.8 pg/ml
样品类型 Serum, plasma (EDTA, heparin) and cell culture supernatants.
标准范围 93.7 - 6000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 5.8%
Inter-Assay CV: 6.4%
別名 ARP; Arginine-rich protein; Protein ARMET; ARMET; Mesencephalic astrocyte-derived neurotrophic factor

应用说明

检测时间 ~ 5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 7873 Human MANF

Swiss-port # P55145 Human Mesencephalic astrocyte-derived neurotrophic factor

基因名称 MANF
全名 mesencephalic astrocyte-derived neurotrophic factor
背景介绍 The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014]
生物功能 Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents in dopaminergic neurons (By similarity). Inhibits cell proliferation and endoplasmic reticulum (ER) stress-induced cell death. [UniProt]
细胞定位 Secreted. Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Note=Retained in the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) under normal conditions (PubMed:22637475). Up-regulated and secreted by the ER/SR in response to ER stress and hypoxia (PubMed:22637475, PubMed:29497057). [UniProt]
产品亮点 Related products:
ARMET antibodies; ARMET ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰 May contain sialic acid residues. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG82404 Human ARMET ELISA Kit standard curve image

    ARG82404 Human ARMET ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82404 Human ARMET ELISA Kit User manual download icon Download