ARG82858
Human AKR1C4 ELISA Kit
Human AKR1C4 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82858-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82858-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82858-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82858-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82858-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82858-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82858-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82858-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82858-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82858-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82858-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82858 Human AKR1C4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AKR1C4 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | AKR1C4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 10 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.6% Inter-Assay CV: 5.9% |
| 別名 | EC 1.1.1.357; CHDR; Aldo-keto reductase family 1 member C4; 3-alpha-HSD1; Chlordecone reductase; EC 1.1.1.225; DD-4; EC 1.1.1.-; HAKRA; 3-alpha-HSD; CDR; 3-alpha-hydroxysteroid dehydrogenase type I; C11; DD4; Dihydrodiol dehydrogenase 4 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P17516 Human Aldo-keto reductase family 1 member C4 |
|---|---|
| 基因名称 | AKR1C4 |
| 全名 | aldo-keto reductase family 1, member C4 |
| 背景介绍 | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008] |
| 生物功能 | Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20-alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 翻译后修饰 | The N-terminus is blocked. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
-
ARG82858 Human AKR1C4 ELISA Kit standard curve image
ARG82858 Human AKR1C4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82858 Human AKR1C4 ELISA Kit User manual |
 Download
|
