ARG21553

Goat anti-Mouse Kappa Light Chain antibody (Biotin), pre-adsorbed

Goat anti-Mouse Kappa Light Chain 抗体 (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Mouse

概述

产品描述 Biotin-conjugated Goat Polyclonal antibody recognizes Mouse Kappa Light Chain
反应物种 Ms
应用 ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性 The antibody reacts with Mouse kappa light chains. The antibody is pre-adsorbed with Mouse lambda light chains, so the antibody may not react with Mouse lambda light chains, but may react with kappa light chains from other species.
宿主 Goat
克隆 Polyclonal
同位型 IgG
靶点名称 Kappa Light Chain
抗原物种 Mouse
偶联标记 Biotin

应用说明

预吸附 Mouse lambda light chains.
应用建议
应用 推荐稀释比
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity purification with immunogen.
缓冲液 PBS and 0.1% Sodium azide.
抗菌剂 0.1% Sodium azide
浓度 0.5 mg/ml
存放说明 Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

检测图片 (4) Click the Picture to Zoom In

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Cytokeratin 18 antibody (right), followed by ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: BALB/c Mouse splenocytes stained with ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) followed by Streptavidin (FITC).

  • ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Cytokeratin 18 antibody (right), followed by ARG21553 Goat anti-Mouse Kappa Light Chain antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.