ARG65668
DefineBlot Mouse anti-Rabbit IgG antibody (HRP)
DefineBlot Mouse anti-Rabbit IgG 抗体 (HRP) for ELISA,Western blot and Rabbit
Immune System antibody
概述
| 产品描述 | HRP-conjugated Mouse Monoclonal antibody recognizes native Rabbit IgG but not denatured Rabbit IgG |
|---|---|
| 反应物种 | Rb |
| 应用 | ELISA, WB |
| 特异性 | ARG65668 DefineBlot Mouse anti-Rabbit IgG antibody (HRP) only detecting native IgG but not denatured IgG is easy to replace traditional secondary antibodies and useful to decrease denatured IgG interference in Co-IP experiment. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 靶点名称 | IgG |
| 抗原物种 | Rabbit |
| 目标Ig | Rabbit IgG |
| 偶联标记 | HRP |
应用说明
| 应用建议 |
|
||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | In Co-IP experiment, denatured IgG from IP samples may interfere target signal in immunoblotting at ~55 kDa (heavy chain) and ~25 kDa (light chain) by using traditional secondary antibodies. ARG65668 DefineBlot Mouse anti-Rabbit IgG antibody (HRP) only detecting native IgG but not denatured IgG is easy to replace traditional secondary antibodies and useful to decrease denatured IgG interference in Co-IP assay. WB: It is suggested using less than 10 μg of primary antibody for IP process, and we also recommend freshly adding 100-200 mM DTT in loading buffer for IP sample and boiling for 5-10 min to completely denature IgG. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS (pH 7.3), 0.01% Thimerosal, 1% BSA, 10% Glycerol and other stabilizers |
| 抗菌剂 | 0.01% Thimerosal |
| 稳定剂 | 1% BSA, 10% Glycerol |
| 浓度 | 0.4-0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 研究领域 | Immune System antibody |
|---|
