ARG80859

Cortisol ELISA Kit

Cortisol ELISA 试剂盒 for ELISA and All

Metabolism kit; Signaling Transduction kit

概述

产品描述 An enzyme immunoassay for the quantification of active free cortisol in saliva.
反应物种 All
应用 ELISA
靶点名称 Cortisol
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm
灵敏度 0.019 ng/ml
样品类型 Saliva.
标准范围 0.1 - 30 ng/ml
样本量 50 µl

应用说明

检测时间 60, 30 min

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

全名 Cortisol
背景介绍 The morning peak always appears during the first 2 hours after the regular wake-up time. During the day the Cortisol concentration decreases and in the evening we can see very low values. This test is especially suited for measuring very low concentration values. If you want to exclude an Addison syndrome please collect saliva during the morning peak. In case of a suspected Cushing's syndrome we are recommending to always use late evening samples as recommended by Nieman et al in JCEM 2008 Vol.93, No.5, pages 1526-1540.

In case of stress testing we are recommending to collect saliva samples during the first 2 hours of a day and another set of samples in the late evening. Then the lab will measure the morning mixture and the evening mixture giving 2 mean results.

In order to avoid arbitrary results we highly are recommending to always take 5 samples during a 2 hour period and measure the average value of the mixture.
Usually the salivary concentration of Cortison is about twice the concentration of Cortisol. This is similar to serum/plasma and urine as well.
产品亮点 Related products:
Cortisol antibodies; Cortisol ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
研究领域 Metabolism kit; Signaling Transduction kit

检测图片 (1) Click the Picture to Zoom In

  • ARG80859 Cortisol ELISA Kit standard curve image

    ARG80859 Cortisol ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80859 Cortisol ELISA Kit User's manual download icon Download