ARG81299

Cell Cycle / Apoptosis Analysis Kit

Datasheet

订购信息

包装

价格 (USD)

200 tests

310

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装
ARG81299-1	Staining Buffer	4 X 20 ml
ARG81299-2	25 X Propidium Iodide (PI)	2 X 1.5 ml
ARG81299-3	RNase A (2.5 mg/ml)	0.8 ml

概述

产品描述	ARG81299 Cell Cycle / Apoptosis Analysis Kit can be used to measure Cell Cycle / Apoptosis activity in cells.
反应物种	Other
应用	FuncSt
靶点名称	Cell Cycle / Apoptosis
偶联标记说明	Excitation: 488 nm.
样品类型	Cells
样本量	50 µl

应用说明

检测时间	~ 3 hours

属性

形式	Liquid
存放说明	Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍	The content of DNA is changed with the process of cell cycle. And the DNA can be stained by fluorescent dye to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1, S, GS/M phase and as well as apoptosis and aneuploidy cells with signals at sub-G1 or super G2 region. Propidium iodide (or PI) is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. Therefore PI is used widely as a DNA dye in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. However, PI also binds to RNA, and it interference with the DNA measurement in the assay. In this Cell Cycle / Apoptosis Analysis Kit we provide an RNase to degrade RNA before PI staining to eliminate the interference by RNA and the kit can be used to monitor cell cycle progression, proliferation and apoptosis by flow cytometry.

背景介绍

The content of DNA is changed with the process of cell cycle. And the DNA can be stained by fluorescent dye to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1, S, GS/M phase and as well as apoptosis and aneuploidy cells with signals at sub-G1 or super G2 region.
Propidium iodide (or PI) is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. Therefore PI is used widely as a DNA dye in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. However, PI also binds to RNA, and it interference with the DNA measurement in the assay. In this Cell Cycle / Apoptosis Analysis Kit we provide an RNase to degrade RNA before PI staining to eliminate the interference by RNA and the kit can be used to monitor cell cycle progression, proliferation and apoptosis by flow cytometry.

检测图片 (1) Click the Picture to Zoom In

ARG81299 Cell Cycle / Apoptosis Analysis Kit functional study image

Functional study: Jurkat cells stained with ARG81299 Cell Cycle / Apoptosis Analysis Kit.