ARG82810
Caspase 1 Activity Assay Kit
Caspase 1 Activity Assay 试剂盒 for Functional study and Human,Mouse,Rat,All
概述
| 产品描述 | ARG82810 Caspase 1 Activity Assay Kit is a detection kit for the quantification of Caspase 1 activity in tissue extracts, cell lysates and other biological fluids. |
|---|---|
| 反应物种 | Hu, Ms, Rat, All |
| 应用 | FuncSt |
| 靶点名称 | Caspase 1 |
| 偶联标记说明 | Read at 405 nm. |
| 检测范围 | 5 - 500 µmol/L |
| 样品类型 | Tissue extracts, cell lysates and other biological fluids. |
| 样本量 | 40 µl |
| 別名 | Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC 3.4.22.36 |
应用说明
| 检测时间 | ~ 1 hour |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CASP1 |
| 全名 | caspase 1, apoptosis-related cysteine peptidase |
| 背景介绍 | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012] |
| 生物功能 | Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related products: Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Related news: RIP1 activation and pathogenesis of NASH |
| 翻译后修饰 | The two subunits are derived from the precursor sequence by an autocatalytic mechanism. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
-
ARG82810 Caspase 1 Activity Assay Kit standard curve image
ARG82810 Caspase 1 Activity Assay Kit results of a typical standard run with optical density reading at 405 nm.
| Title | Download Link |
|---|---|
| ARG82810 Caspase 1 Activity Assay Kit User manual |
 Download
|
