ARG82642
Canine IL10 ELISA Kit
Canine IL10 ELISA 试剂盒 for ELISA and Dog
概述
| 产品描述 | ARG82642 Canine IL10 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Canine IL10 in serum, plasma, cell lysate and tissue lysate. |
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| 反应物种 | Dog |
| 应用 | ELISA |
| 特异性 | The following recombinant canine proteins were tested and exhibited no cross-reactivity or interference: Adiponectin, ApoAI, BMP1, BMP2, BMP3, BMP4, BMP5, BMP7, CCL2, CCL4, CCL5, CRP, HGF, HSP27, IL1 beta, IL1RA, IL1 alpha, IL1 RI, IL2, IL4, IL5, IL6, IL6R, IL8, IL12, IL13, IL15, IL17C, IL21, IL23, IFN beta, IFN gamma, PDGF, PLA2G7, prolactin, serpin E1, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TL3, TLR9, TNF alpha, TNF RI, TNF RII, VEGF and VEGF R1. |
| 靶点名称 | IL10 |
| 偶联标记 | HRP |
| 偶联标记说明 | Read at 450 nm. |
| 灵敏度 | 13 pg/ml |
| 样品类型 | Serum, plasma, cell lysate and tissue lysate. |
| 标准范围 | 25 - 1600 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5% Inter-Assay CV: 8% |
| 別名 | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
应用说明
| 检测时间 | ~ 3.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IL10 |
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| 全名 | interleukin 10 |
| 背景介绍 | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. [provided by RefSeq, May 2011] |
| 生物功能 | Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:1940799, PubMed:7512027, PubMed:11564774). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity). [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: IL10 antibodies; IL10 ELISA Kits; IL10 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82642 Canine IL10 ELISA Kit standard curve image
ARG82642 Canine IL10 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82642 Canine IL10 ELISA kit User manual |
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