ARG81297
AST / Aspartate Transaminase Assay Kit
AST / Aspartate Transaminase Assay 试剂盒 for Functional study and Human,Mouse,Rat,Mammal
参考文献1
概述
| 产品描述 | ARG81297 AST / Aspartate Transaminase Assay Kit is a detection kit for the quantification of AST / Aspartate Transaminase in serum and plasma. |
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| 反应物种 | Hu, Ms, Rat, Mamm |
| 应用 | FuncSt |
| 特异性 | Aspartate aminotransferase (ASAT/AAT) facilitates the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate. And then oxaloacetate and NADH are converted to malate and NAD by malate dehydrogenase. Therefore, the decrease in NADH absorbance at 340 nm is proportionate to AST activity. |
| 靶点名称 | AST / Aspartate Transaminase |
| 偶联标记说明 | Read at 340 nm. |
| 灵敏度 | 2 U/l |
| 检测范围 | 2 - 100 U/l |
| 样品类型 | Serum and plasma. |
| 样本量 | 20 µl |
| 別名 | Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic |
应用说明
| 应用说明 | Please note that this kit does not include a microplate. |
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| 检测时间 | 10 min |
属性
| 形式 | Liquid |
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| 存放说明 | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | GOT1 |
| 全名 | glutamic-oxaloacetic transaminase 1, soluble |
| 背景介绍 | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008] |
| 生物功能 | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81297 AST / Aspartate Transaminase Assay Kit functional study image
Functional study: ARG81297 AST / Aspartate Transaminase Assay Kit results of a typical example of AST activity reading at 340 nm.
文献引用
Investigating the changes of some enzymes and metabolites of the Urea cycle in patients with type 2 diabetes treated with squalene
FuncSt / Human
| Title | Download Link |
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| ARG81297 AST / Aspartate Transaminase Assay Kit User's manual |
 Download
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