ARG23079

anti-Thrombomodulin antibody [QBEND/40]

anti-Thrombomodulin antibody [QBEND/40] for IHC-Frozen sections,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections and Human,Rabbit

Overview

Product Description Mouse Monoclonal antibody [QBEND/40] recognizes Thrombomodulin
Mouse anti Human CD141 antibody, clone QBEND/40 recognizes human CD141, also known as thrombomodulin. Mouse anti Human CD141 antibody, clone QBEND/40 all human endothelium tested, but does not inhibit thrombomodulin dependent activation of protein C.
Tested Reactivity Hu, Rb
Tested Application FACS, IHC-Fr, IHC-P
Host Mouse
Clonality Monoclonal
Clone QBEND/40
Isotype IgG2a
Target Name Thrombomodulin
Antigen Species Human
Immunogen Human endothelium derived thrombomodulin.
Conjugation Un-conjugated
Full Name thrombomodulin
Alternate Names THRM; CD antigen CD141; THPH12; TM; Thrombomodulin; CD141; BDCA3; Fetomodulin; AHUS6

Application Instructions

Application Suggestion
Application Dilution
FACSNeat
IHC-Fr1:10 - 1:100
IHC-P1:10 - 1:50
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0).
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Tissue Culture Supernatant.
Buffer Tissue Culture Supernatant, 0.2M Tris/HCl (pH 7.4), 5-10% foetal calf serum and 0.01% Sodium azide
Preservative 0.01% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 7056 Human THBD

Swiss-port # P07204 Human Thrombomodulin

Gene Symbol THBD
Gene Full Name thrombomodulin
Background The protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq, Jul 2008]
Function Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. [UniProt]
PTM N-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.

Images (8) Click the Picture to Zoom In

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-Fr image

    Immunohistochemistry: Human spleen cryosection stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40]. (Low power).

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-Fr image

    Immunohistochemistry: Human Tonsil cryosection stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40]. (Low power).

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-Fr image

    Immunohistochemistry: Human Tonsil cryosection stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40]. (Medium power).

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-Fr image

    Immunohistochemistry: Human Tonsil cryosection stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40]. (High power).

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-Fr image

    Immunohistochemistry: Human Tonsil cryosection stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40]. (High power).

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-Fr image

    Immunohistochemistry: Human tonsil cryosection stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40], red in A and Mouse anti Human CD21, green in B. C is the merged image with nuclei counterstained blue using DAPI. (Low power).

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-Fr image

    Immunohistochemistry: Human tonsil cryosection stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40], red in A and Mouse anti Human CD21, green in B. C is the merged image with nuclei counterstained blue using DAPI. (High power).

  • ARG23079 anti-Thrombomodulin antibody [QBEND/40] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lymph node stained with ARG23079 anti-Thrombomodulin antibody [QBEND/40].