ARG20305

anti-RFP + dsRed antibody

anti-RFP + dsRed antibody for ICC/IF,Immunoprecipitation,Western blot and Other

Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
publication_link 参考文献1

概述

产品描述 Rabbit Polyclonal antibody recognizes RFP + dsRed
反应物种 Other
应用 ICC/IF, IP, WB
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 RFP + dsRed
抗原 Recombinant RFP expressed from E. coli
偶联标记 Un-conjugated
別名 Red Fluorescent Protein

应用说明

应用建议
应用 推荐稀释比
ICC/IFAssay-dependent
IP10-20 µg/ml
WB0.5-4 µg/ml
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式 Liquid
纯化 Affinity Purified Antibody
缓冲液 PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
抗菌剂 0.01% Thimerosal
稳定剂 30% Glycerol, 0.5% BSA
浓度 0.5 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

背景介绍 Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
产品亮点 Related Antibody Duos and Panels:
ARG30061 Tag Internal Control Antibody Duo (dsRed, GAPDH)
Related products:
RFP antibodies; RFP ELISA Kits; RFP Duos / Panels; Anti-Rabbit IgG secondary antibodies;
研究领域 Controls and Markers antibody; Tag Internal Control antibody; Fluorescent-Tags antibody
预测分子量 4 kDa

检测图片 (1) Click the Picture to Zoom In

  • ARG20305 anti-RFP + dsRed antibody WB image

    Western blot: 1. Recombinant RFP (1000 ng), 2. Recombinant RFP (250 ng), 3. Recombinant RFP (50 ng), and 4. Recombinant RFP (10 ng) stained with ARG20305 anti-RFP + dsRed antibody.

文献引用

A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells.

WB / Other (dsRed)

Murakami Manabu et al.
PLoS One.,  (2019)

publication_link

 

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