ARG66505
anti-PIK3CA / p110 alpha (H1047R mutation) antibody
anti-PIK3CA / p110 alpha (H1047R mutation) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
概述
| 产品描述 | Mouse Monoclonal antibody recognizes PIK3CA / p110 alpha (H1047R mutation) |
|---|---|
| 反应物种 | Hu |
| 应用 | IHC-P, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 同位型 | IgG3, kappa |
| 靶点名称 | PIK3CA / p110 alpha |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide derived from Human PIK3CA / p110 alpha (H1047R mutation). |
| 偶联标记 | Un-conjugated |
| 別名 | MCM; MCMTC; p110alpha; PI3-kinase subunit alpha; PI3K; CWS5; p110-alpha; PI3Kalpha; CLOVE; EC 2.7.1.153; MCAP; PtdIns-3-kinase subunit p110-alpha; EC 2.7.11.1; Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; PI3K-alpha; PtdIns-3-kinase subunit alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA |
应用说明
| 应用建议 |
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| 应用说明 | IHC-P: Antigen Retrieval: Citrate buffer (pH 6.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| 实际分子量 | ~ 150 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purification with immunogen. |
| 缓冲液 | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 50% Glycerol and 0.5% BSA |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P42336 Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha iso |
|---|---|
| 基因名称 | PIK3CA |
| 全名 | phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha |
| 背景介绍 | Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. [provided by RefSeq, Jul 2008] |
| 生物功能 | Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS. [UniProt] |
| 预测分子量 | 124 kDa |
检测图片 (2) Click the Picture to Zoom In
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ARG66505 anti-PIK3CA / p110 alpha (H1047R mutation) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric adenocarcinoma stained with ARG66505 anti-PIK3CA / p110 alpha (H1047R mutation) antibody at 1:200 (4°C, overnight). Antigen Retrieval: Citrate buffer (pH 6.0) was used.
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ARG66505 anti-PIK3CA / p110 alpha (H1047R mutation) antibody WB image
Western blot: 30 µg of K562 whole cell lysate stained with ARG66505 anti-PIK3CA / p110 alpha (H1047R mutation) antibody at 1:1000 dilution.
