anti-Ku80 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
|产品描述||Rabbit Polyclonal antibody recognizes Ku80|
|抗原||Recombinant protein of Human Ku80.|
|別名||double-strand-break rejoining; Thyroid-lupus autoantigen; Nuclear factor IV; KU80; DNA repair protein XRCC5; KARP1; Lupus Ku autoantigen protein p86; EC 3.6.4.-; CTCBF; CTC85; ATP-dependent DNA helicase 2 subunit 2; X-ray repair cross-complementing protein 5; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; KARP-1; X-ray repair complementing defective repair in Chinese hamster cells 5; 86 kDa subunit of Ku antigen; KUB2; NFIV; TLAA; Ku80; Ku86|
|应用说明||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|缓冲液||PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.|
|抗菌剂||0.02% Sodium azide|
|存放说明||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|注意事项||For laboratory research only, not for drug, diagnostic or other use.|
|全名||X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)|
|背景介绍||The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]|
|生物功能||Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. [UniProt]|
|细胞定位||Chromosome, Nucleus, nucleolus. [UniProt]|
|翻译后修饰||Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.
Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites (PubMed:22266820). Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks (PubMed:26502055). [UniProt]
检测图片 (2) Click the Picture to Zoom In
ARG57867 anti-Ku80 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate cancer stained with ARG57867 anti-Ku80 antibody at 1:100 dilution.
ARG57867 anti-Ku80 antibody WB image
Western blot: 25 µg of HepG2 cell lysate stained with ARG57867 anti-Ku80 antibody at 1:1000 dilution.