ARG62653
anti-Tropomyosin (sarcomeric) antibody [CH1]
anti-Tropomyosin (sarcomeric) antibody [CH1] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody
概述
| 产品描述 | Mouse Monoclonal antibody [CH1] recognizes Tropomyosin |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| 特异性 | specific for human striated muscle forms of tropomyosin, including cardiac alpha tropomyosin and skeletal muscle forms of tropomyosin. Reacts with human striated muscle forms of tropomyosin. Not cross-react with smooth muscle or non-muscle isoforms of tropomyosin. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | CH1 |
| 同位型 | IgG1 |
| 靶点名称 | Tropomyosin |
| 抗原物种 | Chicken |
| 抗原 | Purified chicken muscle tropomyosin. |
| 偶联标记 | Un-conjugated |
| 別名 | C15orf13; Tropomyosin alpha-1 chain; TMSA; CMD1Y; HEL-S-265; HTM-alpha; CMH3; Tropomyosin-1; LVNC9; Alpha-tropomyosin |
应用说明
| 应用建议 |
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||||||
| 阳性对照 | Human tongue, heart and chicken heart |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Protein G purified |
| 缓冲液 | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| 抗菌剂 | 0.09% Sodium azide |
| 稳定剂 | 0.2% BSA |
| 浓度 | 0.2 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TPM1 |
| 全名 | tropomyosin 1 (alpha) |
| 背景介绍 | Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types. |
| 生物功能 | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Signaling Transduction antibody |
| 预测分子量 | 33 kDa |
| 翻译后修饰 | Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells. |
检测图片 (1) Click the Picture to Zoom In
-
ARG62653 anti-Tropomyosin (sarcomeric) antibody [CH1] WB image
Western blot: 50 µg of Rat heart, Rat skeletal muscle, Mouse heart, Mouse skeletal muscle, HeLa, U2OS, A549 and Caco-2 cell lysates stained with ARG62653 anti-Tropomyosin (sarcomeric) antibody [CH1] at 0.5 µg/ml, overnight at 4°C.
克隆号文献
eIF4EBP3L acts as a gatekeeper of TORC1 in activity-dependent muscle growth by specifically regulating Mef2ca translational initiation.
Normal myofibrillar development followed by progressive sarcomeric disruption with actin accumulations in a mouse Cfl2 knockout demonstrates requirement of cofilin-2 for muscle maintenance.
Monoclonal antibodies against chicken tropomyosin isoforms: production, characterization, and application.
