anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Try199) antibody

anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Try199) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Immune System antibody; Signaling Transduction antibody
publication_link 参考文献2


产品描述 Rabbit Polyclonal antibody recognizes PI3 Kinase p85 / p55 phospho (Tyr467 / Try199)
反应物种 Hu, Ms, Rat
应用 IHC-P, WB
特异性 The antibody reacts PI3 Kinase p85 and p55 only when phosphorylated at tyrosine 467 of p85 and tyrosine 199 of p55.
宿主 Rabbit
克隆 Polyclonal
同位型 IgG
靶点名称 PI3 Kinase p85 / p55
抗原物种 Human
抗原 Peptide sequence around phosphorylation site of Tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85 / p55.
偶联标记 Un-conjugated
別名 GRB1; PI3-kinase subunit p85-alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; IMD36; PtdIns-3-kinase regulatory subunit alpha; p85-ALPHA; p85; AGM7; PtdIns-3-kinase regulatory subunit p85-alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha


应用 推荐稀释比
WB1:500 - 1:1000
应用说明 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


形式 Liquid
纯化 Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液 PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂 0.02% Sodium azide
稳定剂 50% Glycerol
浓度 1 mg/ml
存放说明 For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项 For laboratory research only, not for drug, diagnostic or other use.



GeneID: 18708 Mouse PIK3R1

GeneID: 25513 Rat PIK3R1

GeneID: 5295 Human PIK3R1

基因名称 PIK3R1
全名 phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
背景介绍 Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
生物功能 Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). [UniProt]
产品亮点 Related products:
PI3K P85 antibodies; Anti-Rabbit IgG secondary antibodies;
研究领域 Cancer antibody; Immune System antibody; Signaling Transduction antibody
预测分子量 84 kDa
翻译后修饰 Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

检测图片 (1) Click the Picture to Zoom In

  • ARG51810 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Try199) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with sorbitol stained with ARG51810 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Try199) antibody.


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