ARG70236

Human TIM3 recombinant protein (ECD) (His-tagged, C-ter)

Human TIM3 recombinant protein (ECD) (His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human

概述

产品描述 HEK293 expressed, His-tagged (C-ter) Human TIM3 recombinant protein (ECD).
反应物种 Hu
应用 Binding, SDS-PAGE
靶点名称 TIM3 (ECD)
物种 Human
A.A. 序列 Ser22 - Arg200 of Human TIM3 (NP_116171.3) with 6X His tag at the C-terminus.
表达系统 HEK293
別名 KIM-3; TIM3; TIMD-3; Tim-3; TIM-3; CD366; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD3; T-cell membrane protein 3; HAVcr-2; T-cell immunoglobulin mucin receptor 3; Hepatitis A virus cellular receptor 2

应用说明

应用说明 Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Galectin9 at 2 ug/ml can bind recombinant human HAVCR2 with a linear range of 0.3-5 ug/ml.

属性

形式 Powder
纯化说明 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
纯度 > 97% (by SDS-PAGE)
缓冲液 PBS (pH 7.4)
复溶 Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
存放说明 For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 84868 Human HAVCR2

Swiss-port # Q8TDQ0 Human Hepatitis A virus cellular receptor 2

基因名称 HAVCR2
全名 hepatitis A virus cellular receptor 2
背景介绍 The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. [provided by RefSeq, Sep 2011]
生物功能 Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand (PubMed:24825777). Regulates macrophage activation (PubMed:11823861). Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance (PubMed:14556005). In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse (PubMed:24337741, PubMed:26492563). In contrast, shown to activate TCR-induced signaling in T-cells probably implicating ZAP70, LCP2, LCK and FYN (By similarity). Expressed on Treg cells can inhibit Th17 cell responses (PubMed:24838857). Receptor for LGALS9 (PubMed:16286920, PubMed:24337741). Binding to LGALS9 is believed to result in suppression of T-cell responses; the resulting apoptosis of antigen-specific cells may implicate HAVCR2 phosphorylation and disruption of its association with BAG6. Binding to LGALS9 is proposed to be involved in innate immune response to intracellular pathogens. Expressed on Th1 cells interacts with LGALS9 expressed on Mycobacterium tuberculosis-infected macrophages to stimulate antibactericidal activity including IL-1 beta secretion and to restrict intracellular bacterial growth (By similarity). However, the function as receptor for LGALS9 has been challenged (PubMed:23555261). Also reported to enhance CD8+ T-cell responses to an acute infection such as by Listeria monocytogenes (By similarity). Receptor for phosphatidylserine (PtSer); PtSer-binding is calcium-dependent. May recognize PtSer on apoptotic cells leading to their phagocytosis. Mediates the engulfment of apoptotic cells by dendritic cells. Expressed on T-cells, promotes conjugation but not engulfment of apoptotic cells. Expressed on dendritic cells (DCs) positively regulates innate immune response and in synergy with Toll-like receptors promotes secretion of TNF-alpha. In tumor-imfiltrating DCs suppresses nucleic acid-mediated innate immune repsonse by interaction with HMGB1 and interfering with nucleic acid-sensing and trafficking of nucleid acids to endosomes (By similarity). Expressed on natural killer (NK) cells acts as a coreceptor to enhance IFN-gamma production in response to LGALS9 (PubMed:22323453). In contrast, shown to suppress NK cell-mediated cytotoxicity (PubMed:22383801). Negatively regulates NK cell function in LPS-induced endotoxic shock (By similarity). [UniProt]
细胞定位 Membrane; Single-pass type I membrane protein. Cell junction. Note=Localizes to the immunological synapse between CD8+ T-cells and target cells. [UniProt]
预测分子量 33 kDa
翻译后修饰 O-glycosylated with core 1 or possibly core 8 glycans.

Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG70236 Human TIM3 recombinant protein (ECD) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70236 Human TIM3 recombinant protein (ECD) (His-tagged, C-ter).